Plasmid_Backbone

Part:BBa_K1441001:Design

Designed by: Reza Alavi   Group: iGEM14_Georgia_State   (2014-10-02)


PGAPz alpha C + Linker


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 765
    Illegal XbaI site found at 788
    Illegal SpeI site found at 803
    Illegal PstI site found at 825
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 765
    Illegal SpeI site found at 803
    Illegal PstI site found at 825
    Illegal NotI site found at 776
    Illegal NotI site found at 814
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 765
    Illegal BglII site found at 1
    Illegal BamHI site found at 1243
    Illegal XhoI site found at 736
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 765
    Illegal XbaI site found at 788
    Illegal SpeI site found at 803
    Illegal PstI site found at 825
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 765
    Illegal XbaI site found at 788
    Illegal SpeI site found at 803
    Illegal PstI site found at 825
    Illegal NgoMIV site found at 2000
    Illegal NgoMIV site found at 2061
    Illegal AgeI site found at 962
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 1226
    Illegal BsaI.rc site found at 1425
    Illegal BsaI.rc site found at 2422
    Illegal SapI site found at 244


Design Notes

we had to design the new MCS the way that would not create stop codon while translating out protein.


Source

no

References